Simultaneous determination of paracetamol and diclofenac in wastewater by high-performance liquid chromatography method

In this study, a simple, selective, and accurate analysis method was developed for the simultaneous determination of anti-inflammatory drugs (paracetamol diclofenac) existing inlet outlet wastewater treatment system. This used solid-phase extraction (SPE) reversed-phase high-performance liquid chromatography with photodiode array detector (HPLC-PDA). Separation performed using an HSR C18 column (250 mm × 4.6 mm; 5 µm) mobile phase consisting acetonitrile: sodium dihydrogen orthophosphate in ratio 60:40 v/v. The flow rate 1 ml/min, PDA detected at 243 nm paracetamol 276 diclofenac. duration diclofenac 2.39 4.39 min, respectively. validated terms accuracy, precision, linearity two found range 0.01 - µg/ml, 0.02 µg/ml paracetamol, diclofenac, limits detection were 0.003 0.006 respectively, whereas quantification µg /ml recoveries greater than 95 %. described also has been applied to determine simultaneously